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제목 |
Mycobacterium lepraemurium in cultured mouse peritoneal macrophage |
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저자 |
Y. T. YANG, S. H. Han, and S. Y. Kim |
소속 |
Dept. of Microbiology College of Medicine, Yonsei University and The World Vision Leprosy Treatment and Research Center, Seoul, Korea |
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년도 |
1968 |
권 |
5 |
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호 |
1 |
번호 |
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시작페이지 |
109 |
끝페이지 |
117 |
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첨부 |
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요약 |
Efforts have been made to accomplish a long term in vitro cultivation of mouse
peritoneal macrophage as a host cell for growth of Mycobacterium lepraemurium.
Following the inoculation with live or heat-killed Myco. lepraemurium of cultured
macrophages either on a cover-slip in Leighton tube or in a small petri dish,
microscopic observations of acid-fast stained slide preparation, and total counts of AF
bacilli that were released by ultrasonic treatment from the macrophages in small petri
dish have been followed in order to present microscopic and quantitative evidences of
the actual multiplication of Myco. lepraemurium in cultured mouse peritoneal
macrophage. The results are summarized and concluded as follows;
1. Successful long term in vitro cultivation of mouse peritoneal macrophage has been
accomplished. The growth media for tissue culture is consisted of NCTC 109;50%, heat
inactivated calf serum; 40% and beef embryo extract (diluted 1 : 5) 10% and the media
was renewed every 3 to 4 days. The incubation temperature was at 37℃ before the in-
oculation with Myco. lepraemurium, and at 30℃ after the inoculation with Myco. leprae-
murium. The CO2 content inside the CO2 humidity incubator
for the cultivation of macrophage was kept at 5%.
2. In cultures of macrophage inoculated with live Myco. lepraemurium, there observed
clear features of incresaes in the number of AF bacilli inside individual cell, elongation
of bacilli and increased solidness in AF staining. However, these features were absent in
cultures of macrophage inoculated with heat-killed Myco. lepraemurium.
3. The ultrasonic treatment of macrophage inoculated with live Myco. lepraemurium
and the quantitative assessment of total number of AF bacilli through the courses of 6
to 8 weeks after inoculation has provided a partial but substantial evidences of actual
multiplication of Myco. lepraemurium in cultured macrophages. |
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내용 |
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