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제목 |
한센병에서의 정량 중합효소 연쇄반응, 조직 및 피부도말 항산균 염색 결과 간의 비교 |
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저자 |
김종필 |
소속 |
한국한센복지협회 |
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년도 |
2015 |
권 |
1 |
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호 |
48 |
번호 |
2 |
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시작페이지 |
17 |
끝페이지 |
30 |
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첨부 |
나학회지2015.pdf |
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요약 |
Comparison of the results of quantitative real-time PCR, and AFB stain of tissue and slit skin smear in Hansen’s disease
Jong-Pill Kim M.D.
Institute for Leprosy Research, Korean Hansen Welfare Association
Abstract
Mycobacterium leprae, the etiological agent of leprosy, is noncultivable on axenic media. Therefore, the viability of M. leprae for clinical or experimental applications is often unknown. To provide new tools for M. leprae viability determination, quantitative reverse transcriptase PCR (RT-PCR) assays were recently introduced and characterized. M. leprae 16S rRNA was used as RNA targets, and M. leprae repetitive element (RLEP) DNA was used to determine relative bacterial numbers in the sample. And introduced the ratio of 16S rRNA and RLEP as the indicator of M. leprae viability for short-term experimental purposes and for predicting M. leprae viability in biopsy specimens to monitor treatment efficacy.
The author studied the comparison of results of quantitative real-time PCR(16S rRNA/RLEP) & AFB stain of slit skin smear & histopathology for evaluating of correlation of them & estimating the viability of M. leprae. There was a correlation between between 16S rRNA/RLEP ratio and BI(r=0.369, p=0.012), and was statistically significant between 16S rRNA/RLEP ratio and histopathological positivity of AFB(p=0.011). However there was no correlation between 16S rRNA/RLEP ratio and MI, it needs the further evaluation the correlation about that.
Key word: slit skin smear, 16S rRNA/RLEP ratio |
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내용 |
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