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제목 한국에서 분리된 나균 rpoB 유전자 돌연변이에 대한 조사
저자 김종필, 김연실 소속 한국한센복지협회 연구원
년도 2007 40
2 번호 1
시작페이지 3 끝페이지 14
첨부 김종필, 김연실.pdf
요약 Global efforts to control leprosy by intensive chemotherapy, including rifampin, have led to a significant decrease in the number of registered patients. But emergence of rifampin-resistant strains of pathogenic mycobacteria has threatened the usefulness of this drug in treating mycobacterial diseases. Mutations in the rpoB gene, encoding the βsubunit of RNA polymerase, were reported to result in resistance to rifampin in several mycobacterial species, including M leprae. To prevent the emergence and transmission of drug-resistant leprosy and to identify and treat existing cases, it is necessary to establish rapid methods for detection of drug resistance in M leprae. However, M. leprae has not been cultivated on artificial media; therefore, to identify drug susceptibility patterns, bacteria must be tested using Shepard's mouse footpad assay. This in vivo method requires at least 6 months and relatively large numbers of bacteria. Recently, there have been advances in the elucidation of molecular events responsible for drug resistance in mycobacteria
Sequences of the rpoB, genes were analyzed for 43 isolates of Mycobacterium leprae from leprosy patients in Korea. Three isolates(6.98%) showed representative mutations in the rpoB, genes, suggesting the emergence of rifampin-resistant M. leprae.

Key words ; leprosy, mutation, rpoB gene