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제목 |
Cuitivation of Mycobacterium Leprae Muriun and Mycobacterium Leprae by the Tissum Culture Method |
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저자 |
In Ja Lee, Shin Yung Hong, and Joon Lew |
소속 |
Department of Nursing and Microbiology, College of Medicine Yonsei University, Seoul, Korea |
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년도 |
1967 |
권 |
4 |
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호 |
1 |
번호 |
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시작페이지 |
91 |
끝페이지 |
102 |
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첨부 |
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요약 |
Since the demonstration of Mycobacterium leprae murium and its recognition as the
causative agent of rat leprosy, this intracellular parasite has been an object of unusual
interest in connection with the problem of human leprosy. Like the human leprosy
bacillus, it has thus far defied all efforts at cultivation in vitro, whether in
bacteriological media or in cell cultures.
Many microbiologists have attempted to do artificial culture of Mycobacterium leprae.
Hansen in 1872, after his finding of Mycobacterium leprae, was the first person to
discover that this microorganism could cause an infectious disease in the human body.
This was 10 years before the discovery of Mycobacterium tuberculosis by Koch. It is
generally known that there are no any successful artificial culture methods of
Mycobacterium leprae at the present time.
In the 1940's, Hanks tried to cultivate the leprosy bacilli by means of the tissue
culture method extensively and systemically but in 1950, according to his report, it was
impossible to cultivate the Mycobacterium leprae.
However, Chang at the National Institute of Health, U.S.A., attempted to culture
Mycobacterium leprae murium with the tissue culture method without regarding the
report of Hanks. In 1960, he reported the multiplication of Mycobacterium leprae murium
in the peritoneal macrophages of mice.
In 1963, Rees 무기 his associates reported that Mycobacterium leprae were cultured in
the abroblasts of mice and rats. Japanese microbiologist Nakamura reported the
multiplication of Mycobacterium leprae murium in the testicular fibroblasts in mice.
To date, there is no validity of Chang's report, therefore we are trying to do similar
experiments to test the validity of the contribution. At the same time attempts were
made to cultivate Mycobacterium leprae murium and Mycobacterium leprae with various
cell cultures of cold blooded animals. |
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내용 |
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