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제목 |
Activity and Influx of Granuloma Macrophages in Human LL |
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저자 |
Gue Tae Chae, Shi Ryong Choi, Sung Jun Choi#, Koo Weon Jeong#, Do I1 Kim#, Sung Hwa Kim## |
소속 |
Chronic Disease Laboratory, #Dept. of Pathology Catholic University Medical College, Korean Leprosy Association, and ##Catholic Clinic+H25 |
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년도 |
1990 |
권 |
23 |
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호 |
1 |
번호 |
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시작페이지 |
12 |
끝페이지 |
28 |
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첨부 |
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요약 |
Granuloma is an immunologic and pathologic unit of human lepromatous leprosy(LL),
which is chiefly composed of macrophages that present absence of specific cell mediated
immunity(CMI) against M. leprae. The failure of the macrophages to kill or inhibit M.
leprae is a conspicuous characteristic of LL. Macrophages isolated from the M.
leprae-infected foot pads of athymic mice are refractory to activation by gamma
interferon. It is likely that killing and or clearing of M. lepra from the granulima must
involve recruitment and turnover of fresh mononuclear phagocytes from bone marrow.
Untill recently influx and turnover of macrophages are not known in spite of its
importance to understanding of human LL granuloma. We examined labelling index(LI),
effects of interferon-γ, interleukin-2, and BCG on microbicidal activity, and
cyclooxygenase pathway metabolites of arachidonic acid including prostaglandin
E2 production of macrophages from untreated human LL granuloma in
vitro. We studied serum prostaglandins contents in the multibacillary and paucibacillary
patients, too.
1. Labelling index of untreated LL granuloma was 14.34±0.63% by autoradiography in
vitro. This means LL granuloma is a high turnover granuloma.
2. Organ culture of treated LL skin showed 5.5% of labelling macrophages in the
dermis.
3. Add of IFN-γ(1,000 units), IL-2(2,000 units), and BCG 2.5×106 to 1×
105 LL granuloma macrophages separately induced partial activation of the
macrophages. This finding supports role of BCG in vaccination of leprosy.
4. Untreated human LL granuloma macrophages produced cyclooxygenase metabolites of
arachidonic acid TXB2, 6-keto-PGF1α, and
PGE2 in order of amount. IL-2, BCG treatment rather than IFN-γ
enhanced PGE2 production in vitro.
5. Serum level of PGE2 seems to be higher in multibacillary patients than
paucibacillary ones. It may be possible that continous follow up assay of
PGE2 before and during treatment will reflect the state of CMI in patients. |
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