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제목 |
Detection of Antibodies to Phenolic Glycolipid Ⅰ of Mycobacterium leprae by Gelatin Particle Agglutination Test for Serodiagnosis of Leprosy |
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저자 |
Sang Nae Cho, Joo Deuk Kim, Hye Kyung Kang#, Do Il Kim#, Sung Hwa Kim##, Soon Bong Suh## |
소속 |
Dept. of Microbiology, Yonsei University College of Medicine, Seoul, Institute for Leprosy Research, Korean Leprosy Control Association, Anyang#, and The Catholic Skin Disease Clinic, Taegu, Republic of Korea## |
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년도 |
1989 |
권 |
22 |
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호 |
1 |
번호 |
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시작페이지 |
50 |
끝페이지 |
60 |
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첨부 |
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요약 |
Phenolic glycolipid Ⅰ(PGL-Ⅰ), a Microbacterium leprae-specific antigen, has been
widely used for the serodiagnosis of leprosy. To date, most of the studies have
employed an enzyme-linked immunosorbent assay (ELISA) for the detection of
antibodies to the antigen. Although ELISA is a sensitive method, it requires expensive
equipments and trained personnel ; therefore, it has been desired to develop further
simple tools which can be implemented in the endemic areas of leprosy.
Recently, an agglutination test using gelatin particles sensitized with a neoglycoprotein,
NT-P-BSA, has been developed. Therefore, this study was initiated to evaluate the
gelatin particle agglutination test (GPAT) kit for detecting anti-PGL-Ⅰ antibodies and
the results obtained by GPAT was compared to those by ELISA. For GPAT, diluted
serum was mixed with an equal volume of sensitized gelatin particles in the wells of
microtiter plates and incubated at room temperature for two hours and the results were
read visually based on the agglutination patterns. Although the instruction of the GPAT
kit indicated four patterns, there were nine identified in this study. Particularly, there
was minor problem in differentiating doubtful patterns from weak positive ones.
GPAT titers obtained by using a semi-automatic diluter for serum dilution were the
same as or insignificantly different from those by using a micropipette in 615(95.1%) of
647 serum specimens examined. However, the reproducibility of GPAT was significantly
better(p<0.05, chi-square test) when the diluter was used . When 283 multibacillary
leprosy patients and 338 healthy controls were examined GPAT gave a sensitivity of
59-83.4% and a specificity of 81.8-95.9% based on the criteria of seropositivity among
the agglutination patterns ant ELISA gave 73.5% and 94.1%, respectively. The GPAT
titers were well correlated to the absorbances at ELISA and the agreement was 86.3%
between the two methods in determining seropositivity and seronegativity.
This study thus showed that GPAT was comparable to ELISA in detecting antibodies
to PGL-Ⅰ and was simple enough to be implemented in a small laboratory in the
endemic areas of leprosy. |
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내용 |
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