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제목 |
Immunohistochemical Study of Langerhans Cells in Lerprosy Using S-100 and OKT 6 Antisera |
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저자 |
Tae Kyung Lee, Doo Chan Moon, Kyung Sool Kwon, Tae Ahn Chung, Tetszi Mitsui# |
소속 |
Dept. of Dermatology, College of Medicine, Pusan National University, Pusan, Korea and Dept. of Pathology, School Medicine, Fukuoka University, Japan# |
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년도 |
1987 |
권 |
20 |
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호 |
1 |
번호 |
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시작페이지 |
41 |
끝페이지 |
47 |
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첨부 |
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요약 |
The monoclonal antibody OKT6 and antisera against S-100 protein have both been
advocated as immunologic markers of Langerhans cells in the skin. S-100 antiserum has
an advantage in its ability to stain Langerhans cells in paraffin tissues. In order to
evaluate whether these antibodies stain equivalent numbers of Langerhans cells in
leprosy skin lesions, authors compared the numbers of Langerhans cells staining with
OKT6 antibody and S-100 antiserum on biopsy specimens from 33 patients with various
kinds of leprosy using immunoperoxidase techniques.
Utilizing OKT6 antibody, greater numbers of positive Langerhans cells were found in
the epidermis in tuberculoid and borderline leprosy than in lepromatous leprosy.
However, these differences were not observed with the S-100 antiserum and, overall,
fewer cells were found as compared with the OKT6 antibody.
Both antibodies also stained the dendritic cells in the dermal granulomas. In TT OKT
6 positive cells were more numerous than S-100; in BT and BB the positive cells were
similar in amount with both antibodies, and in BL and LL the OKT 6 positive cells
were focal or absent but S-100 positive cells were diffusely scattered throughout the
granulomas as in the other types of leprosy.
These results indicate that antiserum to S-100 protein and OKT 6 antibody stain
morphologically similar cells (Langerhans cells), but do not provide comparable results
concerning amount of these cells. |
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내용 |
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