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제목 |
Growth Experiment of Mycobacterium leprae in Cultured Mouse Peritoneal Macrophages |
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저자 |
Yang, Y. T., Lew, J |
소속 |
Dept. of Microbiology, Yonsei University College of Medicine, Seoul, Korea |
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년도 |
1975 |
권 |
9 |
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호 |
1 |
번호 |
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시작페이지 |
45 |
끝페이지 |
57 |
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첨부 |
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요약 |
To grow Myocbacterium leprae in cultured mouse peritoneal macrophages, studies
were made with trypsin-purified Myco. leprae on 1) the dynamics of infection of mouse
peritonal macrophages in vitro with Myco. leprae by intraperitoneal inoculation, 2)
growth experiment of Myco. leprae in cultured mouse peritoneal macrophages by in vivo
infection and in vitro cultivation and 3) the observation of pathological changes in
spleens of mice induced by intraperitioneal inoculation of Myco. leprae. Results are
summarized as follows;
1. Continuing and significant decreases were observed in the numbers of both
acid-fast bacilli in cultured macrophages and of macrophages harboring acid-fast bacilli
by the length of intervals between the time of intraperitneal inoculation of Myco. leprae
and the time of initiation of macrophage culture.
2. No evidence of multiplication of Myco. leprae in the peritoneal macrophages in vivo
was found up to 5 months after intraperitoneal inoculation.
3. With cultures of macrophages made 24 hours and 1 week after intraperitioneal
inoculation of Myco. leprae and maintained in vitro up to f to 3 months, microscopic
examination of the stained preparations of cultured macrophages indicated that an
apparent increase in the numbed of acid-fast bacilli in the macrophages did occur.
4. Quantitative experiment with in vivo infected-in vitro cultured macrophages
resealed certain features of increase in the number of total acid-fast bacill in the
cultured macrophages 7 and 9 week after initiation of the cultures.
5. Pathological changes in the spleens of mice inoculated with Myco. leprae were
mainly degenerative nature in the red pulp. No multiplication of Myco. leprae was
observed in the spleens of mice up to 5 months after intraperitioneal inoculation. |
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내용 |
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