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제목 |
Growth Experiment of Mycobacterium leprae in Cultured Mouse Peritoneal Macrophages |
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저자 |
Yang, Y.T., Lew, J., Cho, S.H., im, J.K |
소속 |
Dept. of Microbiology, Yonsei University College of Medicine Seoul, Korea |
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년도 |
1975 |
권 |
9 |
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호 |
1 |
번호 |
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시작페이지 |
37 |
끝페이지 |
43 |
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첨부 |
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요약 |
A simple and effective procedure is described for semi-purification of viable Myco.
leprae from biopsied lepromatous nodules by trypsinization and high-speed centrifugation.
An unique characteristic of this method is a complete omission of conventional grinding
of homogenization of minced lepromatous tissues prior to purification.
Inoculation of trypsin-purified preparation of Myco. leprae into foot pads and ear lobes
of Korean chipmunk (Tamias sibiricus asiaticus, Gmelin) resulted in apparent increases
in total number of acid-fast bacilli per inoculated tissue 8 and 12 months after
inoculation. |
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내용 |
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