|
|
|
|
|
|
|
|
|
|
제목 |
Antigenic Studies of Mycobacterium Leprae and Mycobacterium Lepraemurium by Immunodiffusion |
|
|
|
|
|
저자 |
Kim. J D., Lew, J. |
소속 |
Yonsei University College of Medicine, Seoul, Korea |
|
|
|
년도 |
1972 |
권 |
8 |
|
|
|
호 |
1 |
번호 |
|
|
|
|
시작페이지 |
23 |
끝페이지 |
37 |
|
|
|
첨부 |
|
|
|
|
요약 |
Since G.A. Hansen discovered M. leprae as a causative organism of human lepresy,
many inrestigtosrs have continued their effects to cultivate M. leprae in vivo. Although
Shepard succeeded in multiplying it by animal inoculation in mouse foot pad, M. leprae
still can not be cultured. An bartificial media. Therefore, immunologic studies of leprosy
lag far behind these on other mycobaeterial infections, and the identification and
establishment of relationships among various antigens associated with mycobacteria have
been the subjects of numerous investigations.
Recently the immunodiffusion methods Eave wren widely used for the antigenic
analysis of mycobacteria. Since Parlett and Youmans applied the agar gel diffusion
technique to identify the antigenic relationship between mycobacteria, many investigators
have developed and applied the immunodiffusion methods to antigenic analysis of M.
leprae and other mycobacteria.
Burrell and Rheins studied antigenicity of lepremin and reported that the old tuberculin
and Bepromin were shown to possess common as well as distinct antigens. Pepys et al.
and Pepys observed that PPD preparation and lepromin had three common precipitating
antigens. Sushida and Hirano, Navalkar et al., and Navalkar detected antibodies in the
sera from leprosy patients against culture filtrates of various mycobacteria and lepromin.
Tuma and Silva obtained positive Precipitating reaction between various mycobacterial
antigens arid rabbit serum immunized with leprosy bacilli.
However, only a few papers have been reported on the specific antigens of M. leprae
because of difficulties in obtaining enough amount of purified leprosy bacilli. Some
investigators suggested that leprosy nodule contained certain specific antigens by the
studies of lepromin, unheated leprosy nodules and tissue culture filtrates of M.
lepraemurium. Rees et al. reported that tissue culture filtrates of M. lepraemurium
contained soluble antigens comparable to those in the culture filtrates of other cultivable
mycobacteria, and they assumed that the intracellularly growing M. leprae and M.
lepraemurium in vivo were equally able to produce soluble antigens which could be
released into the surrounding tissue space or into the blood stream. Abe isolated a
water soluble, heat labile, protein antigen from unheated leprosy nodules, which gave
precipitation only with the rabbit anti-leprosy nodule-extract serum. Those studies
suggested that leprosy module and murine leprosy infected tissues contained certain
specific antigens.
The present paper reports the antigenic analysis of unheated human leprosy nodule
extract, purified leprosy bacilli, murine leprosy infected rat testicle extract and purified
murine leprosy bacilli, and the detection of antibodies in the sera from leprosy patients
against various mycobacterial antigens. |
|
|
|
내용 |
|
 | |
| |
|
|
|
|
|
|
|
|
|
