|
|
|
|
|
|
|
|
|
|
제목 |
A Study of the Pathogenesis of Murine Leprosy Infection of Mice |
|
|
|
|
|
저자 |
Chang, I. K, Yang, Y.T., Lew, J |
소속 |
Dept. of Microbiology, Yonsei University College of Medicine Seoul, Korea |
|
|
|
년도 |
1972 |
권 |
8 |
|
|
|
호 |
1 |
번호 |
|
|
|
|
시작페이지 |
1 |
끝페이지 |
21 |
|
|
|
첨부 |
|
|
|
|
요약 |
Rat leprosy bacillus ,i.e., Mycobacterium lepraemurium, discovered by Stefansky in
1903, has never been successfully grown on bacteriological media, and therefore, animal
inoculation was the method of choice for cultiyation of the organism. Recently, growth
of M. lepraemurium has been observed in tissue culture systems.
The applicability of M. lepraemurium as a study of human leprosy has been
frequently advocated, and accordingly, the organism has been extensively used as a
experimental model in leprosy research.
Among the experimental animals known to be susceptible to infection with M.
lepraemurium, fats have been widely used for animal transmission and extensive studies
have been made on the pathogenesis and pathology of murine leprosy infection of rats.
A variety of routes has been developed for animal inoculation of bf. Bepraemurium, i.
e., subcutaneous, intracutaneous, intratesticular, intramuscular, intraperitoneal, intravenous
and etc..
Numerous reports have been published on the studies of murine leprosy infection of
rats following intraperitoneal inoculation. However, as pointed out by Hanks and
Backerman there were rather wide discrepancies in the results of various investigators.
In contrast to murine leprosy infection of rats, only a limited number of studies has
been made on the experimental infection of the mouse with M. lepraemurium.
Furthermore, no systematic studies have been carried out on the pathogenesis of murine
leprosy infection of mice following intraperitoneal inoculation. The rationales for both
intraperitoneal inoculation of M. lepraemurium into mice and suitability of such infected
mice for the study of pathogenesis of murine leprosy infection are indicated by the
following recent observations,i.e.,1) macrophages are the major host cells for the
facultative and obligate intracellular pathogenic bacteria sucll as M. leprae and M.
lepraemuriuml M. tuberculosis, Brucella and Listeria 2) these exist approximately 6×
106 macrophages inside mouse peritoneal cavity, 3) the turn-over rate of
macrophages in mouse peritoneal cavity is estimated at about 0.1% per hour and the
turn-over time at about 40 days, and 4) all kinds of granulomas are made up of
macrophages derived from circulating monocytes originated from the bone marrow.
By the aid of tissue culture technique and knell-standardized counting of acid-fast
bacilli by pin head method of Hanks, pathogenesis of murine leprosy infection of mice
following intraperitoneal inoculation was investigated in relation to 1) dynamics of
multiplication of M. lepraemurium in peritoneal macrophages in vivo, 2) nature of
splenomegaly and multipliration of M. lepraemurium in the spleen 3) participation of
bone marrow and peripheral circulation in the progress of murine leprosy infection, and
4) involvement of major reticuloendothelial systenl and lymphoid organs. |
|
|
|
내용 |
|
 | |
| |
|
|
|
|
|
|
|
|
|
